Beilstein J. Org. Chem.2024,20, 25–31, doi:10.3762/bjoc.20.4
(Bruker, Jeol) without conversion and then can be subjected to an educational protocol of FT, phase correction (manual or automatic), baseline correction, and signal analysis by peakpicking and integration. Students can interactively observe the effect of the parameters on their spectra as all operations
are recorded as “filters” whose arguments may be reversed or changed afterwards to alter the appearance of the processed spectrum [39].
Alternative to performing peakpicking and integration separately, NMRium provides the ‘ranges’ picking (Figure 2), a combined tool for signal selection, integration
control online if their suggestion is correct.
Building one’s own exercises
The options presented before do not involve the entire potential of NMRium for teaching. Lecturers may want to include peakpicking, or for example, for assignment purposes, ranges/zones picking and the possibility to link signals
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Graphical Abstract
Figure 1:
NMRium.org user interface showing a 2D NMR quiz for an unknown compound with a given molecular form...
Beilstein J. Org. Chem.2020,16, 2087–2099, doi:10.3762/bjoc.16.176
for assessment. With large cohorts of CE-LIF data, peakpicking and peak area calculations still remain a problem for fast and accurate quantitation, despite the presence of internal and external standards to reduce misalignment for the qualitative analysis. The peakpicking and area calculation
; electropherogram; glycosylation; monoclonal antibodies; peakpicking; process development; Introduction
Glycosylation is important for the efficacy and function of a majority of the most dominant biologic drugs currently on the global market. In the case of antibody-based biotherapeutics, the absence of
. This can be caused by a combination of sample complexity, temperature, pH, day of analysis, and other physicochemical fluctuations during the operation of the analysis. Although GU calculation can help solve this for the qualitative analysis, there is still difficulty automating peakpicking due to
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Graphical Abstract
Figure 1:
A single bioreactor run with defined culture conditions for twelve days. (A to B) batch GU calculat...